Glycosylation of hyperthermostable designer cellulosome components yields enhanced stability and cellulose hydrolysis
Enzymatic breakdown of cellulose is a major challenge in the production of second‐generation biofuels. Hyperthermostable enzymes from Caldicellulosiruptor bescii, a very efficient cellulolytic bacterium, were assembled into highly active cellulose deconstruction complexes: designer cellulosomes.
We transformed Ca. bescii to produce designer cellulosomes composed of glycosylated scaffoldins and chimaeric Ca. bescii enzymes. This study demonstrated that glycosylation on these designer cellulosomes improved their thermostability and activity.
Read the Original Article here: https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.15251