Cryo-electron microscopy (cryo-EM) revolutionized structural biology by enabling high-resolution imaging of biomolecules in their native state. This free special issue edited by Panagiotis L. Kastritis features 8 review articles that highlight how cryo-EM is moving beyond static structure determination toward understanding the dynamics, assembly, regulation, evolution, and cellular context of biomolecular complexes, increasingly supported by advanced computational and AI-driven methods.
We invite you to read the full issue here, or view the individual articles at the links below:
Structural biology of ferritin nanocages. Eloise Mastrangelo, Flavio Di Pisa
AAA+ protein unfoldases—the Moirai of the proteome. Stavros Azinas, Marta Carroni
Structural diversity of pyruvate dehydrogenase complexes. Sarah N. Bothe, Rafal Zdanowicz
Mapping the evolution of mitochondrial complex I through structural variation. Dong-Woo Shin, Tingting Chen, James A. Letts
TOR signaling on membranes. Robbie Loewith, Lucas Tafur
Revealing the structure of land plant photosystem II: the journey from negative-stain EM to cryo-EM. Roman Kouřil
Towards better structural models from cryo-electron microscopy data with physics-based methods. Hande Boyaci Selcuk, Gabriella Reggiano, Jacob Robson-Tull, Lichirui Zhang, João P. G. L. M. Rodrigues
Exploring the potential of AlphaFold distograms for predicting binding-induced hinge motions. Büşra Savaş, Ayşe Berçin Barlas, Ezgi Karaca